Medically reviewed by our clinical team · Last reviewed Oct 19, 2026 · How we create and review content
What a testosterone test measures
Most testosterone in your blood is bound to proteins: sex hormone-binding globulin (SHBG) and albumin. A small fraction is "free." According to MedlinePlus, tests can measure:
- Total testosterone: bound plus free; the standard first test
- Free testosterone: the unbound portion, useful when SHBG is abnormal or total testosterone is borderline
- Bioavailable testosterone: free plus albumin-bound, used less often
When to test: timing matters
Testosterone follows a daily rhythm and is highest in the morning. Guidelines from the Endocrine Society and the AUA recommend early-morning testing, repeated on at least two separate days, before diagnosing low testosterone. Acute illness can temporarily lower levels, so avoid testing when you're sick.
How to prepare
- Book an early-morning appointment at your nearest Quest or Labcorp location
- Follow any fasting instructions on your lab order. Some panels include fasting glucose or lipids
- Tell your clinician about every medication and supplement, including biotin, which can interfere with some lab tests
- If you work nights, ask your clinician about the best time to test
Tests often ordered alongside testosterone
- LH and FSH: show whether low testosterone starts in the testes or the pituitary
- SHBG: helps interpret total testosterone
- Prolactin: checked when LH is low or normal with low testosterone
- CBC: a baseline hematocrit, important before TRT
- PSA: for men in age groups where prostate monitoring is recommended
- A1C, lipids, TSH: when symptoms or history suggest metabolic or thyroid issues
Understanding your results
Reference ranges vary by laboratory. The AUA uses total testosterone below 300 ng/dL as a reasonable cutoff to support a diagnosis when symptoms are present. One low result isn't enough. Neither is a "low-normal" number without symptoms. Your clinician interprets results with your symptoms, SHBG, LH/FSH and history.
Where to get tested in Houston
Quest Diagnostics and Labcorp both run patient service centers throughout Greater Houston, from Katy and Cypress to Pearland, Clear Lake and The Woodlands. Use the official locators: Quest · Labcorp. When you start with TRT Houston, we send your lab order electronically and review results with you by video.
After the test
If your results suggest low testosterone, the next step is confirmation and a search for causes. If treatment is appropriate, see how testosterone replacement therapy in Houston works. If results are normal but you still feel off, a broader hormone evaluation may help.
Frequently asked questions
Do I need to fast for a testosterone test?
Testosterone itself doesn't usually require fasting, but your panel may include tests that do. Follow the instructions on your lab order.
Why does my testosterone need to be tested twice?
Levels vary from day to day and can drop temporarily with illness or poor sleep. Guidelines require repeat morning testing to confirm a low result.
How long do results take?
Results are often available within a few business days, depending on the lab and the tests ordered.
Can I use a recent test from my doctor?
Bring it to your consult. Your clinician will check whether it was a morning test and whether it should be repeated.
Sources and further reading
- MedlinePlus (NIH): Testosterone levels test
- Endocrine Society: Testosterone Therapy in Men With Hypogonadism (clinical practice guideline, 2018)
- American Urological Association: Evaluation and Management of Testosterone Deficiency guideline
- MedlinePlus (NIH): Complete blood count (CBC)
- National Cancer Institute: Prostate-Specific Antigen (PSA) test
- Quest Diagnostics: Find a location
- Labcorp: Labs and appointments